Development changes in myristoylated alanine-rich C kinase substrate (marcks) phosphorylation expression in rats hippocampus and cortex with acute multi-cerebral infarction and Qingnao drop pilula neuropretection

Background and purpose: This study was undertaken to investigate the development changes of myristoylated alanine-rich C kinase substrate (MARCKS) phosphorylation expression in acute ischemia hippocampus and cortex, to establish the relationship between these changes and ischemia damage. Methods: The acute multi-cerebral infarction model was established by method of Kaneko. The histopathological analyses was observed by microscope (HE staining). The MARCKS and p-MARCKS protein expression were surveyed by western blot technic and immunohistochemical test method. Results: We found that in all model groups, hippocampus and cortex synchronous changed obvious in histopathology. The expression of the MARCKS and p-MARCKS protein phosphorylation was seen in neuron cytoplasm, cytomembrane. MARCKS and p-MARCKS protein in hippocampus and cortex were expressed in normal group vs. other groups (p<0.01). The changes of MARCKS and p-MARCKS protein expression are identical. After acute ischemic , the expression are all increased, the expression of 1h start heighten, the expression of 24h is supreme, form dynamic changes. The expression of 24h obviously super other group. We gave CTM rats dropping pilula at 24h after ischemia. The damage of CTM neuron have more reckless than the model group and the WM group in histopathology. And the MARCKS、p-MARCKS protein expression were lower than the model group. Conclusions: MARCKS phophorylation, correlate with the occurring and extent neuron damage after acute cerebral ischemia. Signal transduction abnormality maybe the important mechanism of ischemia damage. Qingnao drop pilula could intervene PKC-MARCKS signal transduction system, to turn slower MARCKS phophorylation, and to delay the process of neuron death, can lighten acute cerebral ischemia damage. Correspondence to: Qihui Zhang, Fangxinyuan, Fangzhuang, Fengtai District, Beijing China 100078; E-mail: qihuizhang@vip. sina. com